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Nucleotide sequence and analysis of the Vibrio alginolyticus sucrose uptake-encoding region

Identifieur interne : 003633 ( Main/Exploration ); précédent : 003632; suivant : 003634

Nucleotide sequence and analysis of the Vibrio alginolyticus sucrose uptake-encoding region

Auteurs : Gregory L. Blatch [Afrique du Sud] ; Renate R. Scholle [Afrique du Sud] ; David R. Woods [Afrique du Sud]

Source :

RBID : ISTEX:43C3427E988CA1CBF881954A89E28C53F7D120D6

Abstract

The nucleotide sequence of the Vibrio alginolyticus sucrose uptake-encoding region was determined, and contained two genes, scrA and scrK. The scrA gene encodes an enzyme IISucrose (EIIScr) protein of the phosphoenolpyruvate dependent phosphotransferase system and the scrK gene encodes a fructokinase. The deduced amino acid (aa)_sequence for the V. alginolyticus EIIScr protein was homologous with the EIIScr protein from Streptococcus mutants, Salmonella typhimurium (pUR400 system) and Bacillus subtilis. The deduced aa sequence for the V. alginolyticus fructokinase was homologous with the Escherichia coli enzymes, 6-phosphofructokinase (isoenzyme 2) and ribokinase. Transposon phoA mutagenesis experiments indicated that the EIIScr protein was a membrane-bound protein with a region that extended into the periplasm.

Url:
DOI: 10.1016/0378-1119(90)90408-J


Affiliations:


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